Raf,”normally inactive protein kinase–> has internal regulatory domain, pair of phosphates, it’s tumor suppressor is 14-3-3 (normally keeps it inactive)turned on by binding to Rasgoing to activate kinase cascade(but still no 2nd messengers)When MAPK activated

Raf,”normally inactive protein kinase–> has internal regulatory domain, pair of phosphates, it’s tumor suppressor is 14-3-3 (normally keeps it inactive)turned on by binding to Rasgoing to activate kinase cascade(but still no 2nd messengers)When MAPK activated

it will phosphorylate Ras…come back to turn Raf off”