Benzo(a)pyrene,”when you ingest it, will have a hard time excreting it b/c not water solubleepoxide intermediate reacts very strongly w/ Guanine (makes covalent bond w/ amino group)no enzyme to cleave bondModified Guanine has distorted structure in DNA double helix–if not removed before DNA replication takes place (p53’s job), it’s distortedDNA polymerase will not pair it with C-it will pair it with A (G-A mismatch)Cell keeps G and converts opposite T to C (which is good, but doesn’t always happen)Some cells throw out modified G and replace it with T. Original G on DNA sequence, converted to T(A-T basepair

Benzo(a)pyrene,”when you ingest it, will have a hard time excreting it b/c not water solubleepoxide intermediate reacts very strongly w/ Guanine (makes covalent bond w/ amino group)no enzyme to cleave bondModified Guanine has distorted structure in DNA double helix–if not removed before DNA replication takes place (p53’s job), it’s distortedDNA polymerase will not pair it with C-it will pair it with A (G-A mismatch)Cell keeps G and converts opposite T to C (which is good, but doesn’t always happen)Some cells throw out modified G and replace it with T. Original G on DNA sequence, converted to T(A-T basepair

point mutation)”