Caspase Cascade,”involving interplay between metabolism, especially w/ mitochondria, and the nucleushappens only in presence of P53-p53 first shuts off survival of BCL2, activating expression of Apaf 1 (apoptosome), increases expression of Caspase-9, then increases expression of Caspase 3,-7All inactive, so all p53 is doing is increasing their levels (not activating them yet)p53 increase levels of ribonucleotide reductase (RR) & BAX1 which increase cytosolic levels of dATP and Cyt c, respectivelymoving into apoptosis: RR remaining active, continue rerouting deoxynucleotides to ribonucleotides? Ribodeoxy ATP*all cytochrome c going to leak out in cytoplasm, so mitochondria all deadcytochrome C works with deoxy ATP and now big pool of Apaf1 that p53 madenow activating Apaf1, cytochrome C and deoxy ATP activated (no kinases here…all enzymatic signaling)As we start turning Caspases 3 and 7 on
going to increase levels of ___also activates enzyme like profilin that activates ____”