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Weinberg’s Lab,”used 3T3 mouse cell lineswas first looking for cells that lose contact inhibition (focus) that piled on top of each other (most didn’t)he caused a tumorthen isolated DNA from that tumor, extracted it, then cloned DNA that’s in there. 99.99% of sequence will be mouse, but 1 gene is human. Can identify human DNA and distinguish from mouse DNA by molecular marker (weinberg able to take DNA isolated from human carcinoma cell–>this is how Ras^D first identified oncogene found in this cancer line and was able to transfer 3T3 cells and make them tumoregenic)Could have also changed the selection you use. original carcinoma isolating RasD, losing contact inhibition was first they looked for…can go back to same tumor DNA, keep transfection, instead of losing contact inhibition

Biology

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Telomerase inactivation,”cells become immortalized, but are not cancer cells (mouse cells) –>used to identify human oncogenes*took DNA of human tumors, added them to mouse cell lines that have already been immortalized. They aren’t tumor cells, but still require growth factorsBehavior of cells: telomeres are structures on end of chromosomes that have to be maintained every time cells replicatetelomerase expressed at high level (gets shut off very early (exception is stem cells))normal cells: telomere gets bit shorter each replication (50)In cancer cells: telomerase gets reactivated, but not properly regulated. telomeres get too large. Don’t become senescent; but undergo chromosomal rearrangement (lose p53)*took normal cells, try to very carefully reactivate telomerase to become cancer cells

Biology

Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Telomerase inactivation,”cells become immortalized, but[…]

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