Tag: Microcell Biology

Caspase Cascade,”involving interplay between metabolism, especially w/ mitochondria, and the nucleushappens only in presence of P53-p53 first shuts off survival of BCL2, activating expression of Apaf 1 (apoptosome), increases expression of Caspase-9, then increases expression of Caspase 3,-7All inactive, so all p53 is doing is increasing their levels (not activating them yet)p53 increase levels of ribonucleotide reductase (RR) & BAX1 which increase cytosolic levels of dATP and Cyt c, respectivelymoving into apoptosis: RR remaining active, continue rerouting deoxynucleotides to ribonucleotides? Ribodeoxy ATP*all cytochrome c going to leak out in cytoplasm, so mitochondria all deadcytochrome C works with deoxy ATP and now big pool of Apaf1 that p53 madenow activating Apaf1, cytochrome C and deoxy ATP activated (no kinases here…all enzymatic signaling)As we start turning Caspases 3 and 7 on

Biology

Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Caspase Cascade,”involving interplay between metabolism,[…]

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Weinberg’s Lab,”used 3T3 mouse cell lineswas first looking for cells that lose contact inhibition (focus) that piled on top of each other (most didn’t)he caused a tumorthen isolated DNA from that tumor, extracted it, then cloned DNA that’s in there. 99.99% of sequence will be mouse, but 1 gene is human. Can identify human DNA and distinguish from mouse DNA by molecular marker (weinberg able to take DNA isolated from human carcinoma cell–>this is how Ras^D first identified oncogene found in this cancer line and was able to transfer 3T3 cells and make them tumoregenic)Could have also changed the selection you use. original carcinoma isolating RasD, losing contact inhibition was first they looked for…can go back to same tumor DNA, keep transfection, instead of losing contact inhibition

Biology

Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Weinberg’s Lab,”used 3T3 mouse cell[…]

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