Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Carcinogenicity Vs Mutagenicity,”precise correlationlower the[…]
Read moreCategory: Biology
BIO205
BCL2
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.BCL2 survivor protein; keeps cells[…]
Read moreWeinberg’s Lab,”used 3T3 mouse cell lineswas first looking for cells that lose contact inhibition (focus) that piled on top of each other (most didn’t)he caused a tumorthen isolated DNA from that tumor, extracted it, then cloned DNA that’s in there. 99.99% of sequence will be mouse, but 1 gene is human. Can identify human DNA and distinguish from mouse DNA by molecular marker (weinberg able to take DNA isolated from human carcinoma cell–>this is how Ras^D first identified oncogene found in this cancer line and was able to transfer 3T3 cells and make them tumoregenic)Could have also changed the selection you use. original carcinoma isolating RasD, losing contact inhibition was first they looked for…can go back to same tumor DNA, keep transfection, instead of losing contact inhibition
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Weinberg’s Lab,”used 3T3 mouse cell[…]
Read moreAlfatoxin B1
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Read more“HW: Ribonucleotide reductase (RR) and the mitochondrial pore protein, BAX, are two p53 target genes. What two compondents in the p53-dependent “”life or death”” decision do RR and BAX regulate?”
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.“HW: Ribonucleotide reductase (RR) and[…]
Read moreHW: Weinberg first demonstrated that DNA isolated from human tumor cells could transform mouse 3T3 cells. What properties do transformed 3T3 cells exhibit that distinguish them from non-transformed 3T3 cells?,”Non-contact inhibited, round morphology, do not require growth factors
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.HW: Weinberg first demonstrated that[…]
Read more“Based on the figure below, what can you conclude about the difference between sterigmatocystin (SMC) and dibenz[a,h]anthracene (DBA)?”
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Read moreMissense Mutations,”two consequences: P53 unable to bind to DNA
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Missense Mutations,”two consequences: P53 unable[…]
Read moreMulti-Hit Model,”Cancer is a disease of aging. It’s not a disease that affects the young people (rare)bottom graph: how many days during the life of the mouse it’ll go through while tumor freeMyc mice relatively tumor free after 120 days, then gradual decline in # of tumor free individualsCompared to Ras, finding that mice that harbor Ras oncogene start acquiring tumors much, much earlierIf cross two strains, now have mice that have both oncogenes. Ras operating much further upstream than Myc. When put together, get synergistic effect, causing rapid onset of tumorigenesis
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Multi-Hit Model,”Cancer is a disease[…]
Read more“You’re performing the Ames test on two known carcinogens; Benzo(a)pyrene (BaP) and methyl nitrosourea (MNU). If you treat His-Salmonella with MNU, you generate many His+ colonies. In contrast, if you treat His auxotrophic Salmonella with BaP, you generate no His prototrophs. However, if you first incubate the BaP with a liver extract before adding it to the His auxotroph Salmonella, you generate many His prototrophs. How do BaP and MNU differ?”
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.“You’re performing the Ames test[…]
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