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HW: (Fret Graphs)You observe the following activity profiles upon the addition and subsequent removal of EGF to a mutant cell line. RTK activity of HER1=1; MEK activity=1; MAPK activity=4. Which of the following could explain this result?
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review. HW: (Fret Graphs)You observe[…]
Read moreMAPK,”Mitogen-activated Extracurricular signal-regulated Kinasedual-specificity Kinase (threonine, tyrosine)when activated, will rephosphorylate Rafcomes back and shuts Raf back off, goal is to not let proliferative pathway go any further than it already is(If MAPK can phosphorylate, it will shut off)when active, it forms homodimer sort of like PKA, it phosphorylates actual proteins in the cell, a huge number, only looking at a small subset necessary for the cell to proliferateand can come right to the nucleuswithin the nucleus, cohort of DNA transcription factors already there
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.MAPK,”Mitogen-activated Extracurricular signal-regulated Kinasedual-specificity Kinase[…]
Read more14-3-3
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.14-3-3 tumor suppressor (normally keeps[…]
Read moreRaf,”normally inactive protein kinase–> has internal regulatory domain, pair of phosphates, it’s tumor suppressor is 14-3-3 (normally keeps it inactive)turned on by binding to Rasgoing to activate kinase cascade(but still no 2nd messengers)When MAPK activated
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Raf,”normally inactive protein kinase–> has[…]
Read moreGDP,”(Guanosine di-phosphate)form of G proteingets promoted and released by Ras GEFswitch 2 traps it inside G protein(concentration in cytosol very low, and G proteins have very low affinity for it, but high affinity for GTP–>why we need GEF)(alpha helical Sos displaces switch 2, allowing it to diffuse away
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.GDP,”(Guanosine di-phosphate)form of G proteingets[…]
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Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Ras recruits Raf and binds[…]
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Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.GRB2 protein that binds to[…]
Read moreSos,”(binding site for GRB2 protein)GEF for Ras (which is monomeric G protein)promotes GDP (same thing G coupled protein does)alters the conformations of 2 switch regions to promote GDP releasehas an alpha-helical domain–>displaces switch 2 (pushes down)
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Sos,”(binding site for GRB2 protein)GEF[…]
Read moreHW: How can normal HER2 promote cell proliferation even though it does not directly bind to any extracellular growth factors?,”Answer: None of the abovepromote tumor proliferation for 2 reasons: 1) Her2 can form heterodimers with any other Her family members (1, 3, 4). This is going to sensitize the cell to basically grow at very low extracellular concentrations of EGF that normally wouldn’t be enough to activate Her1 or Her4 homodimers. Her2 coming in and forming and activating a large number of other receptors that would normally be monomeric. The other thing that happens, new insight, when you overexpress Her2
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.HW: How can normal HER2[…]
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