Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Sos,”(binding site for GRB2 protein)GEF[…]
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GRB2
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Read moreRas
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Read moreGDP,”(Guanosine di-phosphate)form of G proteingets promoted and released by Ras GEFswitch 2 traps it inside G protein(concentration in cytosol very low, and G proteins have very low affinity for it, but high affinity for GTP–>why we need GEF)(alpha helical Sos displaces switch 2, allowing it to diffuse away
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.GDP,”(Guanosine di-phosphate)form of G proteingets[…]
Read moreRaf,”normally inactive protein kinase–> has internal regulatory domain, pair of phosphates, it’s tumor suppressor is 14-3-3 (normally keeps it inactive)turned on by binding to Rasgoing to activate kinase cascade(but still no 2nd messengers)When MAPK activated
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.Raf,”normally inactive protein kinase–> has[…]
Read more14-3-3
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Read moreMAPK,”Mitogen-activated Extracurricular signal-regulated Kinasedual-specificity Kinase (threonine, tyrosine)when activated, will rephosphorylate Rafcomes back and shuts Raf back off, goal is to not let proliferative pathway go any further than it already is(If MAPK can phosphorylate, it will shut off)when active, it forms homodimer sort of like PKA, it phosphorylates actual proteins in the cell, a huge number, only looking at a small subset necessary for the cell to proliferateand can come right to the nucleuswithin the nucleus, cohort of DNA transcription factors already there
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Read moreHW: (Fret Graphs)You observe the following activity profiles upon the addition and subsequent removal of EGF to a mutant cell line. RTK activity of HER1=1; MEK activity=1; MAPK activity=4. Which of the following could explain this result?
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.HW: (Fret Graphs)You observe the[…]
Read moreHW: Which of the following possible CFP-donors would only bind to a potential YFP-acceptor when the YFP-acceptor is phosphorylated?,”CFP-GRB2
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.HW: Which of the following[…]
Read moreHW: You observe the following activity/FRET profiles upon the addition and subsequent removal of EGF to a mutant cell line. RTK activity of HER1=1. FRET profile of CFP-GRB2 + YFP-SOS =1. FRET profile of CFP-Raf + YFP-14-3-3 =7. What would be the activity profile for MEK in these cells?
Average Rating 0 out of 5 stars. 0 votes.You must log in to submit a review.HW: You observe the following[…]
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